Biofilms are bacterial colonies that form at interfaces, where bacteria are encased in extracellular polymeric substances (EPS). Biofilms are ubiquitous in both artificial systems and our environment. Here we focus on understanding biofilm growth within a stagnant pool of confined diluted culture of the bacteria. Sporosarcina pasteurii is taken as the model bacterium for this study. The motivation behind the choice of this organism stems from the fact that S. Pasteurii has the unique ability to precipitate calcite inside the host media which has tremendous applications in reservoir and restoration engineering. As the biofilm evolves with time inside the confinement, the dynamics of transport is recorded continuously by an optical microscope and the data processed digitally to gain valuable insights into the bio-physical aspects of the system.

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