Abstract

Does response of a cell depend on the history of the direction of mechanical stimulation? A mechanical force field generated by centrifugal force is applied to the scaffold plane in the present study. To control the cell direction (0, 45, 90 degrees) with respect to the tangential force, striped micro-ridges in 3 directions were machined on the scaffold surface by photolithography technique. The behavior (movement, and deformation) of each mouse myoblast after cultivation in the tangential force field for 5 hours was tracked for 10 hours by time-lapse images. Experimental results show that the velocity of each cell migration tends to increase regardless of two-dimensional projected area on the scaffold by hysteresis of exposure to tangential force field perpendicular to the longitudinal direction of the cell. The experimental results will lead to the elucidation of the effect of the direction of force stimulation on cells.

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