This paper presents a new trajectory planning method based on the improved quintic B-splines curves for a three degrees-of-freedom (3-DOF) cable-driven parallel robot (CDPR). First, the conditions of positive cables’ tension are expressed in terms of the position and acceleration constraints of the end-effector. Then, an improved B-spline curve is introduced, which is employed for generating a pick-and-place path by interpolating a set of given via-points. Meanwhile, by expressing the position and acceleration of the end-effector in terms of the first and second derivatives of the improved B-spline, the cable tension constraints are described in the form of B-spline parameters. According to the properties of the defined pick-and-place path, the proposed motion profile is dominated by two factors: the time taken for the end-effector to pass through all the via-points and the ratio between the nodes of B-spline. The two factors are determined through multi-objective optimization based on the efficiency coefficient method. Finally, experimental results on a 3-DOF CDPR show that the improved B-spline exhibits overall superior behavior in terms of velocity, acceleration, and cables force compared with the traditional B-spline. The validity of the proposed trajectory planning method is proved through the experiments.