A particular turbine cascade design is presented with the goal of providing a basis for high quality investigations of endwall flow under high-speed conditions with unsteady inflow. The key feature of the design is an integrated two-part flat plate serving as a cascade endwall at part-span, which enables a variation of the inlet endwall boundary layer conditions. The new design is applied to the T106A low pressure turbine cascade for endwall flow investigations in the High-Speed Cascade Wind Tunnel of the Institute of Jet Propulsion at the Bundeswehr University Munich. Measurements are conducted under realistic flow conditions (M2th = 0.59, Re2th = 2 · 105) in three cases of varying endwall boundary layer conditions with and without periodically incoming wakes. The endwall boundary layer is characterized by 1D-CTA measurements upstream of the blade passage. Secondary flow is evaluated by five-hole-probe measurements in the turbine exit flow. A strong similarity is found between the time-averaged effects of unsteady inflow conditions and the effects of changing inlet endwall boundary layer conditions regarding the attenuation of secondary flow. Furthermore, the experimental investigations show that all design goals for the improved T106A cascade are met.